We narrowed to 2,840 results for: GFP reporter gene

1. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102889

   Purpose

   PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SOX9-T2A-H2B-EGFP repair template

   Plasmid

   #167972

   Purpose

   SOX9 reporter repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 9 (SOX9 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102887

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

   Plasmid

   #102890

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102891

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

   Plasmid

   #121748

   Purpose

   pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   OLLAS epitope tag-IRES2-eGFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

   Plasmid

   #236148

   Purpose

   Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   H2B (H2BC11 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   E1b minimal promoter with the UTRN promoter region

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97309

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIN40C.SFFV.KRAB-mArid3a.IRES.GFP

   Plasmid

   #169306

   Purpose

   Constitutive overexpression of murine KRAB-Arid3a fusion protein with GFP as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   KRAB-Arid3a (Arid3a Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   SFFV

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SIN40C.SFFV.VP64-mArid3a.IRES.GFP

    Plasmid

    #169313

    Purpose

    Constitutive overexpression of murine VP64-Arid3a fusion protein with GFP as reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    VP64-Arid3a (Arid3a Mouse)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCherryGFP-CoV2-Inframe-stop

    Plasmid

    #177620

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-THRA-RFP-EGFP

    Plasmid

    #228537

    Purpose

    Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

    Depositor

    Markus Schuelke

    Article

    Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

    Insert

    Thyroid Hormone Receptor Alpha (THRA Human)
    

    Tags

    eGFP and tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid

    #50486

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    GFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the GFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

    Plasmid

    #159088

    Purpose

    LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Inserts

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    eGFP
    polyA
    

    Use

    Fluorescent reporter

    Promoter

    1.7kb fabp6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HIV-1-GFP ΔEnv CA E45A

    Plasmid

    #217438

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA E45A

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. HIV-1-GFP ΔEnv CA M66I

    Plasmid

    #217440

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA M66I

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217755

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pKSR1-CA3-GS-EGFP (C-KSR-GS)

    Plasmid

    #217757

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 with GGSSGGGGA linker

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

    Plasmid

    #67932

    Purpose

    Translational reporter - Lox 5'UTR

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (LOX Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

    Plasmid

    #233439

    Purpose

    SFFV driven LIDAR reporter with 2 tandem substrate stem loops

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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