We narrowed to 21,453 results for: eng
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Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.2 TDP-43 NLS1 YFP
Plasmid#84912PurposeMammalian expresion of TDP-43 NLS1 YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTDP-43 (TARDBP Human)
TagsYFPExpressionMammalianMutationK82A, R83A, K84APromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP223
Plasmid#13263DepositorInsertPlac-gam-bet-exo
ExpressionBacterialAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNBU2_erm-TetR-P1T_DP-GH023
Plasmid#90324PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoterDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP223
Plasmid#13263DepositorInsertPlac-gam-bet-exo
ExpressionBacterialAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330 spCas9-mSA
Plasmid#113096PurposepX330 vector carrying spCas9-mSA for gene editing in mammalian cell linesDepositorInsertCAS9-MSA
UseSynthetic BiologyTagsMSA (monomeric streptavidinMutationnoneAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SV-ABAactDA
Plasmid#38247DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsVP16/PYL1
Gal4DBD/ABI*
TagsFlag, Gal4DBD, HA, and VP16ExpressionMammalianMutationD143APromoterSV40 (IRES) and SV40pAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330 spCas9-mSA
Plasmid#113096PurposepX330 vector carrying spCas9-mSA for gene editing in mammalian cell linesDepositorInsertCAS9-MSA
UseSynthetic BiologyTagsMSA (monomeric streptavidinMutationnoneAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SV-ABAactDA
Plasmid#38247DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsVP16/PYL1
Gal4DBD/ABI*
TagsFlag, Gal4DBD, HA, and VP16ExpressionMammalianMutationD143APromoterSV40 (IRES) and SV40pAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via …ExpressionMammalianMutationD10A, H840APromoterCMV and U6Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
Plasmid#47550PurposeCas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion alleleDepositorInsertsCas9
ttTi5605 sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER21-SOX4
Plasmid#51304PurposeExpression of SOX4 after doxycycline administrationDepositorInsertSRY (sex determining region Y)-box 4 (SOX4 Human)
UseLentiviralTagsn/aMutationfull length ORFAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by