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pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pBZS-M.CviPI

Plasmid

#198355

Purpose

Express M.CviPI in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviPI (M.CviPI )

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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Flamindo2

Plasmid

#73938

Purpose

Expresses biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

Flamindo2

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-PCYT2

Plasmid

#81074

Purpose

Expresses PCYT2 in mammalian cells

Depositor

Erin O'Shea

Article

Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

Insert

phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b-csgB

Plasmid

#89582

Purpose

Express csgB curli protein with GS linker and Histaq

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB (csgB )

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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Tom20-mCh-FKBP

Plasmid

#162436

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Tom20-mCh-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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JME4472

Plasmid

#129658

Purpose

URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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BiFC3

Plasmid

#190048

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC4

Plasmid

#190049

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC2

Plasmid

#190047

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172 - TAA)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-ChR2-GFP

Plasmid

#26929

Purpose

AAV-mediated expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGL2_170

Plasmid

#199102

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPYRMandi-ABI

Plasmid

#233649

Purpose

Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pJY3919

Plasmid

#113332

Purpose

Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Expression

Yeast

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Pink Flamindo-p2a-bPAC

Plasmid

#202716

Purpose

Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-jRCaMP1a

Plasmid

#113936

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRCaMP1a

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6

Plasmid

#41596

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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mWasabi-C1

Plasmid

#54743

Purpose

Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mWasabi

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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