We narrowed to 5,945 results for: pcas

1. pGS-21a-PcAad1

   Plasmid

   #163039

   Purpose

   expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

   Depositor

   Jean-Marie Francois

   Article

   Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

   Insert

   aryl-alcohol dehydrogenase
   

   Tags

   GST and His6

   Expression

   Bacterial

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-EcRT-Z3-Hyg

   Plasmid

   #232095

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Z3

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT224_(pCA-tTA2)

   Plasmid

   #36430

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tetracycline transactivator
   

   Expression

   Mammalian

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT-YFP-TetR

   Plasmid

   #59018

   Purpose

   Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

   Depositor

   David Sibley

   Article

   Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

   Insert

   YFP-TetR
   

   Use

   Toxoplasma expression

   Promoter

   alpha-tubulin

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-6His-mISG15

   Plasmid

   #12445

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Tags

   6xHis

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-hPPP3CC-FLAG

   Plasmid

   #179162

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HkCas12a-2AeGFP

   Plasmid

   #123634

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   HkCas12a
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Flp-DOG

   Plasmid

   #80084

   Purpose

   Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   Flp-DOG
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Gal4DBD-GBP2

    Plasmid

    #49439

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP2-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-EGFP-C-IFT81

    Plasmid

    #218732

    Purpose

    Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT81 (IFT81 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pCaMKII-tTAc

    Plasmid

    #172125

    Purpose

    Expression of InteinC-tTAC in CaMKII positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    pCaMKII, tTAc
    

    Use

    AAV

    Promoter

    CaMKII

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG tubulin GFP

    Plasmid

    #66105

    Purpose

    expression of chicken tubulin GFP

    Depositor

    Felicitas Proels, Martin Scaal

    Article

    et al Development. 2015 Feb 15;142(4):665-71.

    Insert

    tubulin
    

    Use

    Chicken expression

    Tags

    EGFP

    Promoter

    CAG

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG vGlut1 GCaMP5G

    Plasmid

    #105008

    Purpose

    Presynaptic calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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