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pTH751-CEN-RLuc/min53maxCFLuc

Plasmid

#38217

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 53 codons of the original FLuc gene wer…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH748-CEN-RLuc/min8maxCFLuc

Plasmid

#38214

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first eight codons of the original FLuc gene …

Promoter

ADH1 and TDH3 (=GDP)

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTH747-CEN-RLuc/min4maxCFLuc

Plasmid

#38213

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first four codons of the original FLuc gene w…

Promoter

ADH1 and TDH3 (=GDP)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-HA

Plasmid

#141320

Purpose

Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

HA

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Mouse Cherry Brie Pooled Library

Pooled Library

#170511

Purpose

Genome-wide knockout library. It is identical to the Brie library #73633, replacing Puromycin resistance with mCherry.

Depositor

Pavel Tolar

Article

Newman et al bioRxiv 2021.03.02.433398

Expression

Mammalian

Species

Synthetic

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only
PCT48

Pooled Library

#170392

Purpose

Barcoded plasmid library

Depositor

Jonathan Weissman

Article

Jones et al Genome Biol. 2020 Apr 14;21(1):92. doi:

Expression

Mammalian

Species

Synthetic

Use

CRISPR and Lentiviral

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only
E.coli_GW-sgRNA_Lib3

Pooled Library

#113136

Purpose

Genome-wide gRNA library for E. coli, sub3

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only
E.coli_GW-sgRNA_Lib4

Pooled Library

#113137

Purpose

Genome-wide gRNA library for E. coli, sub4

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only
E.coli_NC_sgRNA_lib

Pooled Library

#113146

Purpose

Genome-wide gRNA library for E. coli, non-targeting control

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only
E.coli_GW-sgRNA_Lib1

Pooled Library

#113134

Purpose

Genome-wide gRNA library for E. coli, sub1

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only
E.coli_GW-sgRNA_Lib2

Pooled Library

#113135

Purpose

Genome-wide gRNA library for E. coli, sub2

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only
Yeast Inducible CRISPRi Library

Pooled Library

#161829

Purpose

Inducible genome-wide CRISPRi library targeting S. cerevisiae genes with 6-12 guide RNAs per gene.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Expression

Yeast

Species

Saccharomyces cerevisiae

Use

CRISPR

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only
pDSG594

Plasmid

#214221

Purpose

competition strain 10 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG578

Plasmid

#214215

Purpose

integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG602

Plasmid

#214229

Purpose

BAC target plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

AraC, folA, trpC

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG467

Plasmid

#214209

Purpose

Arabinose-induced trpC

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

AraC, trpC

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG597

Plasmid

#214224

Purpose

competition strain 9 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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