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Showing: 1261 - 1280 of 17135 results

  3. pZDonor-U6-myogenin stop codon gRNA

    Plasmid
    #69549
    Purpose
    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Insert
    Myogenin C terminus-targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69549-plasmid-map-sequence-id-122972

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  4. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid
    #114026
    Purpose
    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
    Depositor
    Geert Kops
    Article
    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1
    Insert
    eGFP-STOP-HEC1 (NDC80 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Start codon of Hec1 is mutated to stop codon
    Promoter
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    114026-plasmid-map-sequence-id-221030

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  7. CFP(1-158)Stop in pcDNA1/amp

    Plasmid
    #60892
    Purpose
    This vector attaches CFP(1-158) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    CFP(1-158)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    60892-plasmid-map-sequence-id-112569

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  8. Cerulean(1-158)Stop in pcDNA1/amp

    Plasmid
    #60894
    Purpose
    This vector attaches mCerulean(1-158) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    Cerulean(1-158)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    60894-plasmid-map-sequence-id-112542

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  15. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid
    #127353
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127353-plasmid-map-sequence-id-246311

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  17. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid
    #127355
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127355-plasmid-map-sequence-id-246258

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  19. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158162-plasmid-map-sequence-id-314581

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Showing: 1261 - 1280 of 17135 results