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pcDNA3.1(+)-ExRai-AKAR

Plasmid

#118405

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Short P2A

Plasmid

#164110

Purpose

Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM- Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_CREB1_WT

Plasmid

#82203

Purpose

Gateway Donor vector containing CREB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CREB1 (CREB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBMM42

Plasmid

#13331

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

XRCC4 (XRCC4 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
HA-p73α-pcDNA3

Plasmid

#22102

Depositor

William Kaelin

Article

Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

Insert

tumor protein p73 (TP73 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-human MYCN

Plasmid

#74163

Purpose

Mammalian expression of human MycN

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MycN (MYCN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCold-I-Dsup

Plasmid

#90021

Purpose

To express His-tagged Dsup in bacteria

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

His

Expression

Bacterial

Promoter

cspA

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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