168,238 results

1. minCMV-1xmNG-dPCP

   Plasmid

   #231092

   Purpose

   Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

   Depositor

   John Ngo

   Article

   Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

   Insert

   1xmNG-dMCP
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-syn-jYCaMP1s (AAV1)

   Viral Prep

   #135424-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-syn-jYCaMP1s (#135424). In addition to the viral particles, you will also receive purified pAAV-syn-jYCaMP1s plasmid DNA. Synapsin-driven expression of calcium sensor YCaMP1s. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Promoter

   Syn

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

   Plasmid

   #236421

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (Keap1 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Luciferase-IRES-NrasG12D

   Plasmid

   #60834

   Purpose

   Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   NrasG12D (Nras Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSems LifeAct-StayGold

   Plasmid

   #222947

   Purpose

   Expression of LifeAct tagged with StayGold in mammalian cells

   Depositor

   Jacob Piehler

   Article

   Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

   Insert

   LifeAct
   

   Tags

   StayGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-Flag-APH4-Fibcon-icam

   Plasmid

   #233077

   Purpose

   Expresses synCAM-APH4-Fibcon-icam

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   APH4-FIBCON-ICAM
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS79 (eft-3p::Cas9 + sgRNA)

   Plasmid

   #154839

   Purpose

   Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   sgRNA
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS114

   Plasmid

   #215677

   Purpose

   Empty repair plasmid for ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + loxN + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL Luciferase

    Plasmid

    #120798

    Purpose

    For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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