165,821 results

1. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

   Plasmid

   #106309

   Purpose

   The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
   

   Use

   Tags

   6xflag-3xHaloTag(MtoL)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1569

   Plasmid

   #141841

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF680 (ZNF680 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOSIP-KT (KanR, P21)

   Plasmid

   #45987

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. GLE1

   Plasmid

   #155625

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GLE1 (GLE1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HMGA2+GFP

   Plasmid

   #25405

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   high mobility group AT-Hook 2 (Hmga2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   polyadenylation signal lacking

   Promoter

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pCI-Myc3-HPS4

   Plasmid

   #133931

   Purpose

   Expresses 3xMyc-HPS4 construct in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

   Insert

   HPS4 (HPS4 Human)
   

   Use

   Tags

   3xMyc tag

   Expression

   Mammalian

   Mutation

   Glutamic acid 229 to Glycine; Valine 552 to Methi…

   Promoter

   CMV I.E.

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1-copGFP-T2A-Puro

   Plasmid

   #72263

   Purpose

   Express copGFP and puromycin resistance gene under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   copGFP-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   elongation factor-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

   Plasmid

   #84561

   Purpose

   pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
   

   Use

   Lentiviral

   Tags

   Flag, Suntag, oxBFP, AID (auxin-inducible degron)

   Expression

   Mutation

   Promoter

   UbC

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-mCherry (AAV8)

   Viral Prep

   #114469-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   mCherry

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

10. pEF-BOS-T7-CA150

    Plasmid

    #21924

    Depositor

    Mariano Garcia-Blanco

    Article

    Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

    Insert

    CA150 (TCERG1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only