We narrowed to 1,363 results for: Lipi

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pEE032

Plasmid

#176789

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

401

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML1-G-GECO1.2-ERES

Plasmid

#207144

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Mcoln1 (MCOLN1 Human)

Tags

ER-Export Signal (ERES) and G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

Plasmid

#212112

Purpose

Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSUMO CPTP

Plasmid

#170735

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG R106L-CPTP

Plasmid

#170744

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

R106L

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG K60A-CPTP

Plasmid

#170743

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

K60A

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

Plasmid

#212113

Purpose

Expression of GABARAP split tandem protein with mCherry

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mCherry-GABARAP (GABARAP Discosoma sp., Human)

Tags

mCherry

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSUMO R106L-CPTP

Plasmid

#170737

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

R106L-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

R106L

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO K60A-CPTP

Plasmid

#170736

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

K60A-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

K60A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-Lyn-AktAR2

Plasmid

#125197

Purpose

SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

Lyn-AktAR2

Use

Transposon

Tags

Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLOC-MCOLN1

Plasmid

#131583

Purpose

For expression of human MCOLN1 in mammalian cells, GFP-tagged

Depositor

Sean Morrison

Article

Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9

Insert

MCOLN1 (MCOLN1 Human)

Use

Lentiviral

Tags

turboGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP

Plasmid

#212106

Purpose

Expression of GABARAP split tandem protein with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-Lyn-AktAR2-D

Plasmid

#125198

Purpose

SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

Lyn-AktAR2-D

Use

Transposon

Tags

Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

Expression

Mammalian

Mutation

FoxO1 Thr-24 changed to Val rendering this inacti…

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

Plasmid

#112762

Purpose

express GFP-4EBP1-Rheb15(CAAX mutation)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)

Use

Lentiviral

Tags

Rheb15(CAAX mutant)

Mutation

CAAX mutation to prevent lipid localization

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

Plasmid

#169168

Purpose

Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6X Histidine Tag, TEV cleavage site, mCherry

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-AGK

Plasmid

#23578

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AGK (AGK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only