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pNB812

Plasmid

#60160

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Promoter

RNR1pr

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pmCell-Free_KA2_ZIKV NS2B

Plasmid

#145364

Purpose

Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_MYC

Plasmid

#167715

Purpose

Expresses MYC in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYC (MYC Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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PH-Btk-GFP

Plasmid

#51463

Purpose

Is a biosensor for PIP3

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

BTK (aa 1–177) (BTK Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Tet-ON

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_C_FGFR2

Plasmid

#187751

Purpose

MAC-tagged gene expression of human FGFR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FGFR2 (FGFR2 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

Plasmid

#239948

Purpose

Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD6(L3)

Expression

Bacterial and Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo

Plasmid

#239947

Purpose

Expression of MFSD8 loop 9 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD8(L9)

Expression

Bacterial and Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG

Plasmid

#138566

Purpose

Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA Q75L

Plasmid

#19719

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

GTPase deficient & constitutively active Gl…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA S31N

Plasmid

#19718

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S31N mutant for dominant neg function. NOTE: lo…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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MAC_N_ETV4

Plasmid

#167753

Purpose

Expresses ETV4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ETV4 (ETV4 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_GATA4

Plasmid

#167758

Purpose

Expresses GATA4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

GATA4 (GATA4 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM41_KEAP1_KELCH

Plasmid

#178476

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KEAP1_KELCH (KEAP1 Synthetic, Human)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBG-ttLbUV2

Plasmid

#231386

Purpose

LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

Depositor

Qi-Jun Chen

Article

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

ttLbCas12a Ultra V2

Use

CRISPR

Expression

Plant

Mutation

D156R and E795L

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_N_KLF8

Plasmid

#167773

Purpose

Expresses KLF8 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

KLF8 (KLF8 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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