We narrowed to 24,393 results for: p

1. pET29a(+)::9xhis-Tse5

   Plasmid

   #192962

   Purpose

   Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   Tse5
   

   Use

   Tags

   9xhis + TEV tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

   Plasmid

   #186708

   Purpose

   N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

   Depositor

   Paula Tamagnini

   Article

   Mascia et al Green Chemistry, 24 (2022):6156

   Insert

   alr4766
   

   Use

   Synthetic Biology

   Tags

   His-Tag (6His)

   Expression

   Mutation

   Promoter

   PpsbA2*

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

   Plasmid

   #186710

   Purpose

   N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

   Depositor

   Paula Tamagnini

   Article

   Mascia et al Green Chemistry, 24 (2022):6156

   Insert

   alr4766
   

   Use

   Synthetic Biology

   Tags

   His-Tag (6His)

   Expression

   Mutation

   Promoter

   Ptrc.x.tetO2

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPC-Flag-BirA-Linker-Myc-mRap1

   Plasmid

   #184644

   Purpose

   Retroviral expression of BirA-mRap1

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   BirA-rap1
   

   Use

   Retroviral

   Tags

   BirA FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPR2_+10D+7U

   Plasmid

   #149393

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   7bp inserted upstream, and 10bp inserted downstre…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPR2_+10D+10U

   Plasmid

   #149394

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted upstream and downstream of the IHFp…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPR2_IHF_Mut

   Plasmid

   #149396

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is mutated at key positions needed f…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR2_motif_scram

   Plasmid

   #149397

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Upstream motif recognized by Cas1-2/3 during spac…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-Myo1b motor

   Plasmid

   #187364

   Purpose

   Expresses rat myosin 1b isoform b motor domain labelled with EGFP

   Depositor

   Evelyne Coudrier

   Article

   Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

   Insert

   myosin 1b isoform b motor domain (Myo1b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-Myo1b-Tail

    Plasmid

    #187365

    Purpose

    Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

    Insert

    myosin 1b isoform b Tail domain (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLPC-FLAG.HA.HA.Mm.RAP1.I312R

    Plasmid

    #184643

    Purpose

    Retroviral expressions of mouse RAP1 I312R

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Retroviral

    Tags

    HA FLAG

    Expression

    Mammalian

    Mutation

    I312R

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pb mD3S4-dN

    Plasmid

    #180252

    Purpose

    P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

    Depositor

    Timothy Springer

    Article

    Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

    Insert

    D3 of P. berghei HAP2 with N-glycosylation sites mutated
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    N516T, S533N and N539Q

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSC32

    Plasmid

    #104806

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from AtUBQ10 promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g101090
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSC31

    Plasmid

    #104805

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g101090
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSC27

    Plasmid

    #104801

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr4g020620
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSC26

    Plasmid

    #104800

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from AtUBQ10 promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g101040
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSC20

    Plasmid

    #104794

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from AtUBQ10 promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g020630
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSC14

    Plasmid

    #104788

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g044580
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSC15

    Plasmid

    #104789

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g044580
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSC13

    Plasmid

    #104787

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g044580
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge