We narrowed to 9,896 results for: plasmids 101

1. pXint129

   Plasmid

   #178208

   Purpose

   Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

   Depositor

   Gregory Phillips

   Article

   Platt et al Plasmid. 2000 Jan . 43(1):12-23.

   Insert

   xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacI

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. piCRg Entry

   Plasmid

   #58904

   Purpose

   Cas9/gRNA Entry expression plasmid

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFOX6-Tier1-PhCMV-TdTomato

   Plasmid

   #169515

   Purpose

   Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven dimeric red fluorescent protein TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VV201: ChR2(C128S)-eGFP in fubi

   Plasmid

   #58510

   Purpose

   Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   ChR2(C128S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Cysteine 128 to Serine

   Promoter

   Ubiquitin

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVH232-Tier1-PmPGK1-mRuby2

   Plasmid

   #169523

   Purpose

   Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric red fluorescent protein mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJUMP27ts[mCherry]-1A(lacZ)

   Plasmid

   #126980

   Purpose

   Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   RepA A56V thermosensitive mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMM545-Tier1-PhCMV-Citrine

   Plasmid

   #169505

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven yellow fluorescent protein citrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAB303-Tier1-PhCMV-TetR_VPR

   Plasmid

   #169575

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB301-Tier1-PhCMV-rTetR_VPR

   Plasmid

   #169579

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   reverse tetracycline-controlled transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVH227-Tier1-OTetO7-PCMVmin2-SEAP

    Plasmid

    #169563

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP production
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFOX13-Tier1-PhCMV-tGFP

    Plasmid

    #169510

    Purpose

    Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven turbo green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHBJT011

    Plasmid

    #225162

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBJT008

    Plasmid

    #225159

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBJT007

    Plasmid

    #225158

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBJT014

    Plasmid

    #225165

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHBJT013

    Plasmid

    #225164

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBJT012

    Plasmid

    #225163

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBJT010

    Plasmid

    #225161

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBJT009

    Plasmid

    #225160

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBJT006

    Plasmid

    #225157

    Purpose

    Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge