163,774 results

1. dCas-hHDAC3-R265P

   Plasmid

   #103786

   Purpose

   Expresses dCas9 fused to human HDAC3 with R265P mutation

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCas9-HDAC3-R265P (HDAC3 Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed arginine 265 to proline

   Promoter

   EF1A

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBE-T1.14

   Plasmid

   #193277

   Purpose

   Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.14
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYU-2-Env

   Plasmid

   #133997

   Purpose

   Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

   Depositor

   Himanshu Garg

   Article

   Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

   Insert

   Envelope Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPLICS LY-MT Short P2A

   Plasmid

   #213614

   Purpose

   Detect the short-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-flag-GFP-Intb1

   Plasmid

   #205193

   Purpose

   This vector encodes of a synCAM (Intb1) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM Intb1 and GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule2 - Mj Acd A9

   Plasmid

   #197652

   Purpose

   E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

   Depositor

   Ryan Mehl

   Article

   Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

   Inserts

   Mj Acd A9 synthetase
   Methanocaldococcus jannaschii tRNA
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32A, L65D, F108L, Q109E, D158S, L162T

   Promoter

   GlnS and lpp

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTnos (GB0037)

   Plasmid

   #68188

   Purpose

   Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Nos terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-Rncp-iGluSnFR1

   Plasmid

   #107336

   Purpose

   Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

   Insert

   Rncp-iGluSnFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJet1.2 cat cassette

   Plasmid

   #117119

   Purpose

   Chloramphenicol resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Chloramphenicol resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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