We narrowed to 2,957 results for: BFP

1. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221075

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221076

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221077

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221079

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]

   Plasmid

   #133324

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of RRM in full-length TDP43

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]

   Plasmid

   #133326

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of NTD in full-length TDP43

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

   Plasmid

   #159798

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

   Plasmid

   #216478

   Purpose

   lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   YAP5SA
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

   Plasmid

   #216479

   Purpose

   lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   wtYAP (YAP1) (YAP1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

    Plasmid

    #233445

    Purpose

    PiggyBac donor plasmid to overexpress a full length ADAR2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    ADAR2-T2A-PuroR-P2A-BFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187956

    Purpose

    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158651

    Purpose

    making transgenics expressing a UAS driven YFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187957

    Purpose

    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid

    #187953

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

    Plasmid

    #164038

    Purpose

    Expression of dCas9-14xGFP11 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    dCas9-14xGFP11
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    dSV40

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

    Plasmid

    #216480

    Purpose

    lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    TAZ4SA (WWTR1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Mutation

    S66A, S89A, S117A, S311A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

    Plasmid

    #118594

    Purpose

    Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    sgRNA/Puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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