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We narrowed to 3,154 results for: BFP

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Showing: 1301 - 1320 of 3154 results

  3. L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244171
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    IL10Ra signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244171-plasmid-map-sequence-id-487917

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  4. L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244174
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    VEGFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244174-plasmid-map-sequence-id-487918

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  5. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244178
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    TNFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244178-plasmid-map-sequence-id-487910

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  6. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244177
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    TNFR2 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244177-plasmid-map-sequence-id-487904

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  16. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid
    #223106
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223106-plasmid-map-sequence-id-447626

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  17. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid
    #223107
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223107-plasmid-map-sequence-id-447623

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  18. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid
    #223108
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223108-plasmid-map-sequence-id-447631

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  19. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid
    #223109
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223109-plasmid-map-sequence-id-447628

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Showing: 1301 - 1320 of 3154 results