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Plasmid#242676PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8.8m-eVRQR-P2A-EGFP (BKS974)
Plasmid#242655PurposeCMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with neVRQR(D10A) and P2A-EGFPDepositorInsertpCMV-ABE8.8m-VRQR(S55R)-P2A-EGFP
UseCRISPRTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.8 mutations in TadA, eVRQR mutations in SpCa…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)
Plasmid#242675PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-eVRQR-P2A-EGFP (LLH575)
Plasmid#242654PurposeCMV promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with neVRQR(D10A) and P2A-EGFPDepositorInsertpCMV-ABEmax-VRQR-S55R-P2A-EGFP
UseCRISPRTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE7.10 mutations in TadA, eVRQR mutations in SpC…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)
Plasmid#242681PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)
Plasmid#242672PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)
Plasmid#242674PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)
Plasmid#242680PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium OPEN Accessory Reagent Set
Plasmid Kit#1000000013PurposePlasmids and strains used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method.DepositorApplicationGenome EditingVector TypeBacterial Expression, Insect Expression, Mammalian Expression, Plant ExpressionEditing TypeZFNAvailable SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLIA EnR (CC#29)
Plasmid#15152DepositorInsertEngrailed repressor (en Fly)
UseRetroviralExpressionMammalianMutationengrailed repressor domainAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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TetO-FUW-MCS-EnR_C-term
Plasmid#136885PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codonDepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-haA3A-CBE-G-N
Plasmid#220898PurposeAAV genome encoding the N-terminal of haA3A-CBE-GDepositorInserthaA3A-CBE-G-N
UseAAVExpressionMammalianAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-G-NG
Plasmid#220897PurposeExpress haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-G-NG
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-G
Plasmid#220894PurposeExpress haA3A-CBE-G (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-G
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-VA
Plasmid#220893PurposeExpress haA3A-CBE-VA (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-VA
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-A-NG
Plasmid#220895PurposeExpress haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-A-NG
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-A
Plasmid#220892PurposeExpress haA3A-CBE-A (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-A
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-haA3A-CBE-G-C
Plasmid#220899PurposeAAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNADepositorInserthaA3A-CBE-G-C
UseAAVExpressionMammalianAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only