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Plasmid#134863PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30 SUMO-1 G97
Plasmid#13381DepositorInsertSUMO-1, G97 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationG97 mutant: lacks 4 amino acids from the C-termin…PromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFH6
Plasmid#86555PurposeSubcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiencyDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR; SubcloningTagsExpressionMutationPromoterArabidopsis U6-26 promoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1d
Plasmid#134906PurposeExpression of rat Myo 1d (Myr 4) in mammalian cells.DepositorInsertMyo 1d (rat) with 3' UTR (Myo1d Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
optoPKAW196R/Y204A
Plasmid#104546PurposeExpresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.DepositorInsertCry2-mCherry-PKA W196R/Y204A
UseTagsmCherryExpressionMammalianMutationPKA W196R and Y204APromoterCMVAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_TGON2
Plasmid#111677PurposeMAC-tagged gene expressionDepositorInsertTGON2 (TGOLN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH6_new
Plasmid#105866Purposemore efficient version of pFH6; Subcloning of any sgRNA via BbsI siteDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR and Synthetic Biology; SubcloningTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCX-Lyn-SponGee
Plasmid#134776Purposemammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertLyn-SponGee
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus wGBD
Plasmid#85222PurposeVenus tagged Cdc-42 sensorDepositorInsertwGBD (WASL Human)
UseTagsVenusExpressionMammalianMutationCdc42 binding domain of N-WASPPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Dead
Plasmid#173073PurposeDead SNAP tag at the centrosomeDepositorInsertmCherry-SNAP-SNAP-Varied-PACT
UseTagsmCherry-SNAP-SNAP-VariedExpressionMammalianMutationAA144 in both SNAP tags changed from a cysteine t…PromoterCMV IE94Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJR2956
Plasmid#122533PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE49 pt Beta-catenin-myc
Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationfour point mutations introduced into the GSK-3 ph…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJR2955
Plasmid#122550PurposeExpression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemNeptune2.5
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
optoPKAW196R
Plasmid#104548PurposeExpresses Cry2-mCherry-PKA W196R in mammalian cellsDepositorInsertCry2-mCherry-PKA W196R
UseTagsmCherryExpressionMammalianMutationPKA W196RPromoterCMVAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-E2
Plasmid#175242PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylationDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PSPC1-CT
Plasmid#124374Purposeexpresses EGFP-PSPC1-CT131 in mammalian cellDepositorInsertPSPC1 CT (PSPC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-GPXC71S
Plasmid#191703PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701DepositorInsertchl-redox(ro)GFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPP1_native+
Plasmid#67865Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form)DepositorInsertMRPP1 (TRMT10C Human)
UseTagsExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only