Addgene

We narrowed to 12,117 results for: Vars

Showing: 1301 - 1320 of 12117 results

Results per page:

Sort results by:

P21-sp64 Poly A

Plasmid

#59433

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 21

Use

Makes synthetic transcripts that contains poly a

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221-DDX5(PrD)

Plasmid

#166167

Purpose

Gateway entry vector for N. furzeri DDX5-PrD(535-634) mutant

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5-PrD(535-634)

Use

Gateway entry vector

Tags

Expression

Mutation

PrD only 1606-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-DDX5ΔPrD

Plasmid

#166164

Purpose

Gateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutant

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Use

Gateway entry vector

Tags

Expression

Mutation

deletion of 1606-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-DDX5ΔIDR

Plasmid

#166165

Purpose

Gateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutant

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Use

Gateway entry vector

Tags

Expression

Mutation

deletion of 1450-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-DDX5(IDR)

Plasmid

#166166

Purpose

Gateway entry vector for N. furzeri DDX5-IDR(483-634) mutant

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5-IDR(483-634)

Use

Gateway entry vector

Tags

Expression

Mutation

IDR only 1450-1902

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GAL-mRuby3

Plasmid

#166141

Purpose

mRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-ccdB-SNAP

Plasmid

#166146

Purpose

Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Use

Tags

His10 and SNAP

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-Smt3-ccdB

Plasmid

#166147

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Use

Tags

His10-Smt3

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-Smt3-ccdB-SNAP

Plasmid

#166148

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Use

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-Smt3-DDX5(kf)-SNAP

Plasmid

#166149

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Use

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

Plasmid

#166150

Purpose

Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Use

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔPrD(1-535)

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

Plasmid

#166151

Purpose

Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Use

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔIDR(1-483)

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mTln1-F2F3-P229L-pET151

Plasmid

#177879

Purpose

Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mTln1-F2F3-P229S-pET151

Plasmid

#177878

Purpose

Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Serine (P229S)

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSJ982

Plasmid

#176622

Purpose

Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

Ub-MPGLW-Fbp1

Use

Tags

Ubiquitin N-terminal fusion and triple flag tag C…

Expression

Yeast

Mutation

Changed first 4 N-terminal amino acid residues of…

Promoter

TDH3tc3

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD400

Plasmid

#163107

Purpose

Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_eYFP_H-NSdbd_NLS

Use

Eukaryotic expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter; T7 promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD403

Plasmid

#163108

Purpose

Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mScarlet-I_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD128

Plasmid

#163097

Purpose

Expression of mTurquoise2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mTurquoise2_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD129

Plasmid

#163098

Purpose

Expression of mEos3.2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEos3.2_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD188

Plasmid

#163099

Purpose

Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_H-NSdbd_NLS

Use

Eukaryotic expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter; T7 promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge