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pTRANS_210d

Plasmid

#91109

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb720-HO

Plasmid

#141201

Purpose

Expresses mRhubarb720 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb720
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, L196Q, T202D, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-eSIBR

Plasmid

#71664

Purpose

EGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backbone

Depositor

Philip Washbourne

Article

Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

Insert

EGFP coding sequence followed by eSIBR cassette

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_100

Plasmid

#91198

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFP.R270

Plasmid

#138219

Purpose

Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-IRES-puro-polyA

Plasmid

#43967

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Use

Gateway dest vector

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-sgRNA(siteA)

Plasmid

#154831

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteA)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTK503

Plasmid

#114717

Purpose

Express mCherry-NuMA(WT) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5C6A-J23106-geneIII

Plasmid

#80905

Purpose

Phagemid (encodes cI5C6A and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_211

Plasmid

#91110

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMT-3P2A

Plasmid

#111772

Purpose

Quadcistronic construct: Co-express four genes by 3 P2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_231

Plasmid

#91120

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1910

Plasmid

#91041

Purpose

Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTK511

Plasmid

#114686

Purpose

Express Mem-BFP-iLID

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

Mem-BFP-iLID

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAN-NOR

Plasmid

#62306

Purpose

synthetic circuit: NOR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PPhlF and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

Plasmid

#216664

Purpose

Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

Depositor

Leonardo Morsut

Inserts

ETV2 (Etv2 Mouse)
BFP
HygromycinR

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6G,P-J23106-geneIII

Plasmid

#80861

Purpose

Phagemid (encodes evolved cI5G6G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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