163,983 results

1. pLentiV2-dCas9-VP64

   Plasmid

   #141104

   Purpose

   Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

   Depositor

   Igor Ulitsky

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. UBC plasmid

   Plasmid

   #169746

   Purpose

   Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsYTHDF3_AB

   Plasmid

   #148313

   Purpose

   Mammalian Expression of HsYTHDF3

   Depositor

   Elisa Izaurralde

   Insert

   HsYTHDF3 (YTHDF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-RNaseHI-NES-EGFP

   Plasmid

   #196701

   Purpose

   Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   bacterial RNaseHI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Plasmid

   #163056

   Purpose

   mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Grx1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-SFFV-GLuc-CreERT2-mCherry

   Plasmid

   #178185

   Purpose

   the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.

   Depositor

   Irmela Jeremias

   Article

   Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:

   Inserts

   GLuc
   CreERT2
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Membrane mCherry

   Plasmid

   #210470

   Purpose

   Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

   Depositor

   Amro Hamdoun

   Insert

   LCK membrane mCherry (LCK Synthetic, Human, A.victoria)
   

   Use

   Synthetic Biology; Sea urchin, mammal, zebrafish

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA GFP10-RHOA

   Plasmid

   #133068

   Purpose

   Expresses RHOA GTPase with tripartite split GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

   Insert

   ras homolog family member A (RHOA Human)
   

   Use

   Tags

   GFP10 (split-GFP tripartite)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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