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pBad-HisB-GRvT

Plasmid

#87363

Purpose

Expresses GRvT, a tandem heterodimer green and red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GRvT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJH181.3

Plasmid

#135076

Purpose

Coryne. glutamicum HA production plasmid

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Insert

hasA-hasC

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pWT PolI

Plasmid

#11721

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I (polA E. coli)

Expression

Bacterial

Mutation

wild-type

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pBbAk-w4-loxP-TT-loxP-mCherry-PCB

Plasmid

#228469

Purpose

Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

Depositor

Mary Dunlop

Article

Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

Inserts

w4-loxP-TT-loxP-mCherry
Ho1
PcyA

Expression

Bacterial

Promoter

J23106, J23108, and T7A1

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMK392 (mAID-mClover-BSD)

Plasmid

#121193

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-mClover-BS

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pT2SC

Plasmid

#59382

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTTP1A

Plasmid

#91721

Purpose

integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA

Depositor

Graham Hatfull

Article

Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23

Type

Empty backbone

Use

Integration-proficient plasmid vectors for mycoba…

Available Since

May 17, 2017

Availability

Academic Institutions and Nonprofits only

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Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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