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We narrowed to 4,351 results for: Pam

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Showing: 1321 - 1340 of 4351 results

  1. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  2. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  3. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  4. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  5. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  6. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108860-plasmid-map-sequence-id-209932

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  7. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108860-plasmid-map-sequence-id-209932

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  8. SpRYc

    Plasmid
    #175575
    Purpose
    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Article
    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:
    Insert
    SpRYc
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    PID of SpRY grafted onto the N-terminal domain of…
    Promoter
    CMV
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    175575-plasmid-map-sequence-id-345065

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  9. SpRYc

    Plasmid
    #175575
    Purpose
    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Article
    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:
    Insert
    SpRYc
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    PID of SpRY grafted onto the N-terminal domain of…
    Promoter
    CMV
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    175575-plasmid-map-sequence-id-345065

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  14. PB-PE SpRY_ATH51

    Plasmid
    #235998
    Purpose
    Expresses SpRY_ATH51 prime editor and pegRNA in mammalian cells
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    SpRY_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    bpSV40 NLS
    Expression
    Mammalian
    Mutation
    ectopic insertion of 7 amino acids of PID turn-he…
    Available Since
    Aug. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    235998-plasmid-map-sequence-id-470119

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  15. PB-PE SpRY_ATH51

    Plasmid
    #235998
    Purpose
    Expresses SpRY_ATH51 prime editor and pegRNA in mammalian cells
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    SpRY_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    bpSV40 NLS
    Expression
    Mammalian
    Mutation
    ectopic insertion of 7 amino acids of PID turn-he…
    Available Since
    Aug. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    235998-plasmid-map-sequence-id-470119

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  16. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  17. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  18. pYPQ166-SpRY

    Plasmid
    #161520
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9
    Use
    CRISPR; Gateway compatible zsprycas9 entry clone
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161520-plasmid-map-sequence-id-325640

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  19. pYPQ166-SpRY

    Plasmid
    #161520
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9
    Use
    CRISPR; Gateway compatible zsprycas9 entry clone
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161520-plasmid-map-sequence-id-325640

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Showing: 1321 - 1340 of 4351 results