We narrowed to 2,218 results for: dcas9

1. pMOD8A-RGR-r10

   Plasmid

   #74370

   Purpose

   This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NAX_CAGGS_CAS9_m4_VPR_Puro

   Plasmid

   #167887

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRRNA-G20-R20

   Plasmid

   #101822

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF:iCas9

   Plasmid

   #138267

   Purpose

   Expression of mTN3-GGS6-dCas9 (iCas9) in human.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mTN3-GGS6-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cas9-D10A, H840A

   Promoter

   Ef1a

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMOD8A-iRGR-r11

   Plasmid

   #74369

   Purpose

   This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r11
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AHD1

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NAX_CAGGS_CAS9_m4_VPR_EYFP

   Plasmid

   #167882

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas2F

   Plasmid

   #82377

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRRNA-G0-R0

   Plasmid

   #101803

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NAX_CAGGS_CAS9_m4_EYFP

    Plasmid

    #167866

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NAX_CAGGS_CAS9_m4_KRAB_EBFP2

    Plasmid

    #167873

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas2D

    Plasmid

    #82375

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRRNA-G0-R20

    Plasmid

    #101818

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRRNA-G20-R0

    Plasmid

    #101807

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHelper-CRISPRi-sgRNA

    Plasmid

    #221135

    Purpose

    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with dCas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDA010.188

    Plasmid

    #206792

    Purpose

    J23113.dCas9

    Depositor

    James Carothers

    Article

    Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    J23113

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GB2880

    Plasmid

    #193117

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1aG2b.6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GB3277

    Plasmid

    #193131

    Purpose

    A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1abc.4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GB2888

    Plasmid

    #193126

    Purpose

    A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1ab.4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GB2886

    Plasmid

    #193124

    Purpose

    A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1ab.3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge