We narrowed to 4,804 results for: HRE

1. pCAG-3xGFP11-mem-3xPax7gRNA

   Plasmid

   #224571

   Purpose

   Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   3xGFP11
   

   Use

   CRISPR

   Tags

   Membrane Localization Signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2BT-His-TEV-cs-LC3b

   Plasmid

   #197460

   Purpose

   Bacterial Expression of human LC3b

   Depositor

   James Hurley

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   LC3b (MAP1LC3B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF_ApyADO

   Plasmid

   #226248

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF_ApyAO

   Plasmid

   #226242

   Purpose

   Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyO
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF_ApyAD

   Plasmid

   #226241

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2 EGFP sgRNA4

   Plasmid

   #164934

   Purpose

   Expresses EGFP sgRNA4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2 NT sgRNA5

   Plasmid

   #164930

   Purpose

   Expresses Non-targeting sgRNA5 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA5 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LacI-pLacI-pI pm

   Plasmid

   #218098

   Purpose

   Phagemid that expresses LacI, and pI under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMX-IG-ATG3 (H262A)

   Plasmid

   #212027

   Purpose

   Expresses untagged ATG3 (H262A) in mammalian cells

   Depositor

   Michael Lazarou

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

    Plasmid

    #174897

    Purpose

    ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sf GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAA in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1533_pAAV-CB-AcGFP-BirA-HA

    Plasmid

    #208201

    Purpose

    Control for FKBP12-BirA construct

    Depositor

    Susan Hamilton

    Article

    Lee et al Commun Biol. 2023 Sep 14;6(1):942. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Chicken beta actin

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC5GGv1_ShBle

    Plasmid

    #188603

    Purpose

    pSC5GGv1 with Sh ble insert for yeast selection

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTP-PCR C3

    Plasmid

    #189620

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Removed repeat region, 10-kbp deletion, and minor…

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. M2C5

    Plasmid

    #188606

    Purpose

    Minimal Conjugative Plasmid 2

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. M1C1

    Plasmid

    #188609

    Purpose

    Minimal Conjugative Plasmid 1

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACE-K2P4.1-StrepTagII

    Plasmid

    #191474

    Purpose

    Expresses K2P4.1 with C-terminal StrepTagII

    Depositor

    Arthur Laganowsky

    Article

    Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

    Insert

    K2P4.1 (KCNK4 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    N104Q N108Q

    Promoter

    polH

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACE-GIRK2-mC-S

    Plasmid

    #172428

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Use

    Tags

    mCherry Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 LIC 6A CARD8-FLAG F405G

    Plasmid

    #169956

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 CARD8 FIIND L370G

    Plasmid

    #169970

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    L370G

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 CARD8 FIIND L368G

    Plasmid

    #169969

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    L368G

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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