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pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

Plasmid

#234846

Purpose

3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Article

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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HRE-dUnaG

Plasmid

#201711

Purpose

Hypoxia responsive promoter driving destablized UnaG fluorescent protein

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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HRE-UnaG

Plasmid

#201710

Purpose

Hypoxia responsive promoter driving UnaG fluorescent proteins

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

HRE-mCMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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HRE-dUnOHR

Plasmid

#201714

Purpose

Hypoxia responsive promoter driving UnaG and mOrange

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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TOPO HRE

Plasmid

#68458

Purpose

TOPO construct expressing HRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Hypoxia Response Element

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHREAC

Plasmid

#134908

Purpose

Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

double 35S

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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4xHRE_RE:RedF

Plasmid

#178314

Purpose

HRE RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

HRE RedFirefly reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHRE

Plasmid

#134909

Purpose

Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

double 35S

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Article

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Use

Tags

cMyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
TB:4xHRE_RE:MiniP

Plasmid

#178313

Purpose

4 copies of the HRE DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

4 copies of the heat shock DNA binding motif

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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GW1-pHRed

Plasmid

#31473

Purpose

genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pRsetB-pHRed

Plasmid

#31472

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Thomas Foster, Martin Brown

Article

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Insert

5X HRE of VEGF (VEGFA Human)

Use

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

3 copies of FRE

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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FUGW-pHRed

Plasmid

#65742

Purpose

Lentiviral vector for pHRed expression in mammalian cells using the hUbiC promoter

Depositor

Mathew Tantama

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

pHRed

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbiC

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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TRIN-shREN

Plasmid

#105586

Purpose

Dox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only
pUB-spHRed

Plasmid

#84734

Purpose

Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

pHRed

Use

Tags

RbcS targeting sequence

Expression

Plant

Mutation

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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