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pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220127

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

HA tag

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN

Plasmid

#220128

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Tags

HA tag

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

Plasmid

#220129

Purpose

Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSauri Cas9C

Use

AAV and CRISPR

Expression

Mammalian

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

Plasmid

#226915

Purpose

Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSp Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

Plasmid

#226916

Purpose

Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSp Cas9C

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC

Plasmid

#213535

Purpose

Expresses the split transcription factors in cultured neuron and mouse brain

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

Synapsin

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO

Plasmid

#205464

Purpose

A backbone containing a RsDddA fragment B for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-B; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO

Plasmid

#205466

Purpose

A backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-D; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1085L

Plasmid

#191376

Purpose

pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting GFP

Expression

Insect

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1093B

Plasmid

#194003

Purpose

pBac-U6-crCHIKV-array-UTR-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting CHIKV

Expression

Insect

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1163A

Plasmid

#194001

Purpose

pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

Uniq-CasRx (Codon optimized)

Expression

Insect

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1050T

Plasmid

#191374

Purpose

pBac-AedesUbiq-RfxCas13d-p10-Opie2-dsRed.

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

Uniq-CasRx

Expression

Insect

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1085F

Plasmid

#191375

Purpose

pBac-U6-crYellow-array-UTR-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting yellow

Expression

Insect

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pJJGL003

Plasmid

#180607

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX with R3 loop

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -MTalpha-FliG

Plasmid

#154361

Purpose

Bacterial expression of Mtalpha

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Mtalpha

Expression

Bacterial

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -MTn-FliG

Plasmid

#154360

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Expression

Bacterial

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+-AFP

Plasmid

#154359

Purpose

Bacterial expression of AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

AFP

Tags

His

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -2AFP-FliG

Plasmid

#154362

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Expression

Bacterial

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+-2MT

Plasmid

#154353

Purpose

Bacterial expression of 2MT

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2MT

Tags

His

Expression

Bacterial

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+-2AFP

Plasmid

#154358

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Tags

His

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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