Addgene

We narrowed to 1,515 results for: SIR

Showing: 1341 - 1360 of 1515 results

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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Use

Tags

6FLAG

Expression

Yeast

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOME0207

Plasmid

#178407

Purpose

KSHV pre-miRNA K12-2wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0208

Plasmid

#178408

Purpose

KSHV pre mir K12-4wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0209

Plasmid

#178409

Purpose

KSHV pre microRNA K12-5wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME210MR

Plasmid

#178410

Purpose

KSHV pre microRNA K12 6wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0211

Plasmid

#178411

Purpose

KSHV micro RNA K12-7wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0136MR

Plasmid

#178399

Purpose

KSHV Kaposinclouster_RevCompl-KapATGdel_Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Kaposinclouster_RevCompl-KapATGdel_Syn

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0213

Plasmid

#178413

Purpose

KSHV pre microRNA K12-10wt

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K 12

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pQadd1F

Plasmid

#135660

Purpose

Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Intron containing lox site

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

pFer

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pQadd1R

Plasmid

#135661

Purpose

Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Intron containing lox site

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

pFer

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS Exon Vector

Plasmid

#69884

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS Exon Vector

Plasmid

#69893

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-CALPAIN-7-mCherry

Plasmid

#180652

Purpose

Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL7085

Plasmid

#167249

Purpose

orthogonal mRFP reporter output for cell-free expression

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-mRFP1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTK47_mCherry-LGN-hardened

Plasmid

#46347

Purpose

rescues the phenotypes caused by LGN RNAi

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

LGN (GPSM2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-SETin-FLAG-HA

Plasmid

#24999

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations introduced to prevent siRNA targ…

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only