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pCGS652

Plasmid

#62220

Purpose

Mammalian expression TALEN vector with N152, C+18 truncations

Depositor

Daniel Voytas

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Type

Empty backbone

Use

TALEN

Tags

AcV5 and SV40 NLS

Expression

Mammalian

Promoter

miniCaggs

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET-21a-GFP-Patronin CC 534–868

Plasmid

#59054

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM113

Plasmid

#40159

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N (par-3, par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa1-364)

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
ΔCT163-GFP

Plasmid

#33116

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 163 aa of the variable cytop…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
ΔCT183-GFP

Plasmid

#33117

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 183 aa of the variable cytop…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pFM107

Plasmid

#40153

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SA (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SA; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pFM103

Plasmid

#40149

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, wild-type (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

tac

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM109

Plasmid

#40155

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, C56S (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM108

Plasmid

#40154

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, S241A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM106

Plasmid

#40152

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R163A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM105

Plasmid

#40151

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, K162A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM104

Plasmid

#40150

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R183-5A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded …

Promoter

T7

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-hygro-mTurquoise2-Nuf2

Plasmid

#80762

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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BFP-KDEL

Plasmid

#49150

Purpose

visualization of the ER

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

BiP ER signal sequence (HSPA5 Human)

Tags

KDEL and TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAa-DN

Plasmid

#109526

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAa

Plasmid

#109525

Purpose

Muscle specific zebrafish expression of mKate2 fused to RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab6a

Plasmid

#109652

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab6a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6a (rab6a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab6ba

Plasmid

#109653

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab6ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6ba (rab6ba Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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