We narrowed to 152,655 results for: Des

1. pDEST mfap4:turquoise2

   Plasmid

   #135218

   Purpose

   Cyan tagged macrophages

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   mfap4 promoter and mTurquoise2
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti4/V5-DEST-P85

   Plasmid

   #40219

   Depositor

   Lynda Chin

   Insert

   P85 alpha (PIK3R1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLenti6/V5-DEST-MTHFD2

   Plasmid

   #31213

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   MTHFD2 (MTHFD2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2/v5-DEST hGlut1

   Plasmid

   #18085

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut1 (SLC2A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 373 pTol2-fli1ep:CherryDest

   Plasmid

   #73493

   Purpose

   Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signal

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   fli1ep:mCherry-attR1-attR2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti Dest BlastR R1-R2

   Plasmid

   #84574

   Purpose

   Gateway destination vector expressing Blasticidin resistance under a PGK promoter

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

   Plasmid

   #124903

   Purpose

   Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

   Depositor

   Markus Covert

   Article

   Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

   Insert

   p65 promoter- p65 CDS-mRuby2
   

   Use

   Lentiviral

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti6/V5-DEST-NCAPH

    Plasmid

    #31214

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NCAPH (NCAPH Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST14-Cys-SpyCatcher002

    Plasmid

    #102829

    Purpose

    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Cys-SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Cysteine residue in N-terminal region

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 372 pTol2-fli1ep:EGFPDest

    Plasmid

    #73491

    Purpose

    Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    fli1ep:EGFP-attR1-attR2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti6/V5-DEST-RINT1

    Plasmid

    #40124

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    RINT1 (RINT1 Human)
    

    Use

    Lentiviral

    Tags

    V5 epitope

    Expression

    Mammalian

    Mutation

    L270M relative to NM_021930

    Promoter

    CMV

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-AdEvolve-DEST

    Plasmid

    #131692

    Purpose

    A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Jose Dinneny, Cesar Cuevas-Velazquez

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK082 desmaMCS:minprommCherry,exorh:mCerulean

    Plasmid

    #200015

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST53-LRRK2-R1441C

    Plasmid

    #25046

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Arginine 1441 mutated to Cysteine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pDEST40-2XHA-wtSrc

    Plasmid

    #140294

    Purpose

    Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti6/V5-DEST-HMGB1

    Plasmid

    #31208

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pGEX6P1-DEST-His

    Plasmid

    #119763

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    10xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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