Addgene

We narrowed to 47,608 results for: CAL

Showing: 1361 - 1380 of 47608 results

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Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Use

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pNuc-TrxRFP1

Plasmid

#98998

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and nuclear localization sequence

Use

Tags

His-tag and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ11445

Plasmid

#183629

Purpose

pHR-R(ARE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBp-Control Sponge (CXCR4 sponge)

Plasmid

#22744

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
LOVS1K

Plasmid

#31981

Purpose

Expresses a LOVS1K, a light-controllable synthetic protein to modulate local or global Ca2+ signaling

Depositor

Kevin Truong

Article

Pham et al Chem Biol. 2011 Jul 29;18(7):880-90.

Insert

LOVS1K

Use

Tags

RFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV, p10

Available since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pBMFB2

Plasmid

#31278

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 May 27. ():.

Insert

Bmal1 (Bmal1 Mouse)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
PCx35IL-pET42

Plasmid

#42916

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 35 cytoplasmic loop

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 101-179

Promoter

T7

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCKFB1

Plasmid

#31280

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 May 27. ():.

Insert

Clock (Clock Mouse)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pBAD-pnGFP-Ultra

Plasmid

#157923

Purpose

Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra, a high performance peroxynitrite sensor

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FKB

Plasmid

#12499

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

FKB

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pC1BA

Plasmid

#31368

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 May 27. ():.

Insert

Cry1 (Cry1 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
6xHis-MBP-huPcCas12a

Plasmid

#174681

Purpose

Expresses 6xHis-MBP tagged PcCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPcCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPdCas12a

Plasmid

#174682

Purpose

Expresses 6xHis-MBP tagged PdCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPdCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Rad C249

Plasmid

#11228

Depositor

Ronald Kahn

Article

Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

Insert

Rad (RRAD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C249: deletion of amino acids 249-308

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB c-Crk II

Plasmid

#15000

Depositor

Raymond Birge

Article

Zvara et al Oncogene. 2001 Feb 22. 20(8):951-61.

Insert

CrkII (CRK Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only
pIS1-miR-31 site

Plasmid

#25021

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-31 site

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
PL2

Plasmid

#12395

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL2

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
PL1

Plasmid

#12394

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL1

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pBC1FG

Plasmid

#31346

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 May 27. ():.

Insert

Cry1 (Cry1 Mouse)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pBABE Rad N88

Plasmid

#11218

Depositor

Ronald Kahn

Article

Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

Insert

Rad (RRAD Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N88: deletion of amino acids 1-88

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only