We narrowed to 10,497 results for: ESP

1. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

   Plasmid

   #224534

   Purpose

   A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-IRES-mTagBFP2-pA (JDW 1361)

   Plasmid

   #224535

   Purpose

   A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG-NLS-mTagBFP2-pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME H2B mCerulean (JDW 1150)

   Plasmid

   #224543

   Purpose

   A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-mCerulean
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E 5X C120 c-fos min pro (JDW 1241)

   Plasmid

   #224544

   Purpose

   A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   5x-C120-c-Fos promoter
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-NeoCOVGT3-d2EGFP-mCherry

   Plasmid

   #201450

   Purpose

   Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

   Depositor

   Gaetano Gargiulo

   Article

   Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

   Insert

   COVGT3_d2EGFP-mCherry
   

   Use

   Synthetic Biology

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-Neo-COVGT1-d2EGFP-mCherry

   Plasmid

   #201451

   Purpose

   Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

   Depositor

   Gaetano Gargiulo

   Article

   Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

   Insert

   COVGT1_d2EGFP-mCherry
   

   Use

   Synthetic Biology

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-Neo-COVGT2-d2EGFP-mCherry

   Plasmid

   #201452

   Purpose

   Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

   Depositor

   Gaetano Gargiulo

   Article

   Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

   Insert

   COVGT2_d2EGFP-mCherry
   

   Use

   Synthetic Biology

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-Neo-COVGT4-d2EGFP-mCherry

   Plasmid

   #201453

   Purpose

   Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

   Depositor

   Gaetano Gargiulo

   Article

   Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

   Insert

   COVGT4_d2EGFP-mCherry
   

   Use

   Synthetic Biology

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid

    #162950

    Purpose

    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    

    Use

    Synthetic Biology

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

    Plasmid

    #224488

    Purpose

    Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Luciferase

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. NLS-PyronicSF/pCMV-myc-nuc

    Plasmid

    #124814

    Purpose

    Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Tags

    Nuclear Localization Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

    Plasmid

    #224501

    Purpose

    Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin B Vhh mNeonGreen-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

    Plasmid

    #229812

    Purpose

    A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mCherry
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

    Plasmid

    #159579

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

    Plasmid

    #224489

    Purpose

    Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Sun1-2xsfGFP-6xMYC-pA
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Plasmid

    #159580

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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