We narrowed to 2,026 results for: cas9 expression vector

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MS2-P65-HSF1_GFP

Plasmid

#61423

Purpose

Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGLOW39

Plasmid

#173068

Purpose

mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

mScarlet-I-C1^SEC^3xMyc

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mScarlet

Expression

Worm

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L

Plasmid

#89610

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-1

Plasmid

#133353

Purpose

human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_CMV-AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246048

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9; contains ITRs for AAV packaging with Rep2

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-3

Plasmid

#133403

Purpose

human ETV6 gRNA-3 is a 20-nt gRNA expression plasmid targeting the second ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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JN202: pMVP/SB/Blast-DEST

Plasmid

#121864

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JN302: pMVP/SB/Puro-DEST

Plasmid

#121865

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK001: pMVP/SB/Hygro-DEST

Plasmid

#121867

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT601: pMVP/SB/mCherry-DEST

Plasmid

#121862

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK901: pMVP/SB/Zeo-DEST

Plasmid

#121868

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC001: pMVP/PB/Hygro-DEST

Plasmid

#121876

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LB601: pMVP/PB/eGFP-DEST

Plasmid

#121870

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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