We narrowed to 7,176 results for: cas9 plasmid

1. pHEE-R-kan5A9si

   Plasmid

   #222836

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TC9pAB

   Plasmid

   #71310

   Purpose

   used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9
   

   Use

   In vitro transcription

   Tags

   NLS

   Expression

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHEE-R-kan9si

   Plasmid

   #222833

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-hL1-5'_3.3kb plasmid

   Plasmid

   #226006

   Purpose

   Plasmid used to generate DNA-FISH probe targeting at human L1HS

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   Human L1 element 3.3kb sequences at the 5' end
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDCC6

   Plasmid

   #59985

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Inserts

   Cas9
   U6-2>sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   U6-96Ab and hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

   Plasmid

   #68347

   Purpose

   AAV plasmid expressing the FlpO recombinase. with empty gRNA

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   FlPO
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TC9nickpAB

   Plasmid

   #71311

   Purpose

   used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9 nickase
   

   Use

   In vitro transcription

   Tags

   NLS

   Expression

   Mutation

   D10A nickase

   Promoter

   T7

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

   Viral Prep

   #73178-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Promoter

   Tags

   None

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep

    #73633-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Promoter

    Tags

    None

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pT7-module1

    Plasmid

    #222838

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-module2

    Plasmid

    #222839

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-module3

    Plasmid

    #222840

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-module4

    Plasmid

    #222841

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-module5

    Plasmid

    #222842

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 5)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-module6

    Plasmid

    #222843

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 6)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFREE

    Plasmid

    #92050

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFREE_Zeo

    Plasmid

    #92053

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJB06

    Plasmid

    #190480

    Purpose

    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Cas9
    XylR
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    xylR-driven promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge