We narrowed to 66,514 results for: des.1
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Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianPromoterMSCV LTR promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-mCherry (AAV8)
Viral Prep#137132-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-mCherry (#137132). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-mCherry plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre and Flp-dependent)Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE
Plasmid#162374PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD287 (mKate2^SEC^3xMyc
Plasmid#70685PurposemKate2^SEC^3xMyc vector with ccdB sites for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized mKate2ExpressionWormAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP
Plasmid#55641PurposeFlp-Dependent EYFPDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVExpressionMammalianPromoterEf1aAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-2xdBroccoli
Plasmid#66843PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
ExpressionBacterialPromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mCherry-53BP1
Plasmid#127658PurposeOver-express mCherry-53BP1 fragment to monitor 53BP1 foci.DepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsmCherryMutationNucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP (AAV9)
Viral Prep#55641-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Flp-dependent)Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)
Viral Prep#112677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.synP.DIO.EGFP.WPRE.hGH (AAV9)
Viral Prep#100043-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.synP.DIO.EGFP.WPRE.hGH (#100043). In addition to the viral particles, you will also receive purified pAAV.synP.DIO.EGFP.WPRE.hGH plasmid DNA. Synapsin-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFPL10a
Plasmid#98747PurposeCre-dependently expresses the fusion protein EGFPL10aDepositorHas ServiceAAV Retrograde and AAV5InsertEGFPL10a
UseAAV and Cre/LoxTagsGFPExpressionMammalianPromoterEF1aAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterMaize ubiquitin 1 (ZmUbi1)Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHA-mLRH-1
Plasmid#16342DepositorAvailable SinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
GW1CMV-Perceval
Plasmid#21737Purposecan be used to monitor the ATP:ADP ratio during live-cell imagingDepositorInsertPerceval (fluorescent ATP:ADP sensor)
Tags5'UTRExpressionMammalianMutationimproved Kozak sequenceAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY (AAV9)
Viral Prep#45185-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsTagBFP, EYFP, or none.Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-p53-shRNA-941
Plasmid#25637DepositorAvailable SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-miR21
Plasmid#21114DepositorInsertmiR-21 (MIR21 Human)
ExpressionMammalianAvailable SinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
FCK-Halo-GFP
Plasmid#14750PurposeLentiviral expression of Halo-GFP for neuronal inhibitionDepositorInsertMammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
UseLentiviralTagsGFPExpressionMammalianMutationN. pharaonis halorhodopsin is mammalian codon-opt…PromoterCaMKIIaAvailable SinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 E
Plasmid#141273PurposeGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorHas ServiceCloning Grade DNAInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable SinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits