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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,255 results for: tTA

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Showing: 1361 - 1380 of 6255 results

  1. A1-LCD allW

    Plasmid
    #234609
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW (HNRNPA1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) with all aromatic ami…
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234609-plasmid-map-sequence-id-469038

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  3. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

    Plasmid
    #204620
    Purpose
    Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.
    Depositor
    Akitsu Hotta
    Article
    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175
    Insert
    crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
    Use
    CRISPR
    Promoter
    U6
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204620-plasmid-map-sequence-id-404750

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  11. A1-LCD-6R+6K

    Plasmid
    #178670
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-6R+6K (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    six Arg residues replaced with Lys
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178670-plasmid-map-sequence-id-356194

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  13. A1-LCD-3R+3K

    Plasmid
    #178669
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-3R+3K (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    three Arg residues replaced with Lys
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178669-plasmid-map-sequence-id-356159

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  14. A1-LCD+7F-7Y

    Plasmid
    #178660
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD +7F-7Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Tyr residues replaced with Phe
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178660-plasmid-map-sequence-id-356154

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  20. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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Showing: 1361 - 1380 of 6255 results