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pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAG_ToeRep_N09_trigger

Plasmid

#132740

Purpose

T7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRep_N09_trigger

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-C4-Nrf2

Plasmid

#21549

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

EGFP and Myc

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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hAAVS1 TALEN Left

Plasmid

#52341

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Left

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

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pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P1TDPGH023

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAG413GAL-ccdB

Plasmid

#14141

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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mCherry-Lifeact-7

Plasmid

#54491

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

mCherry-Lifeact

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

Plasmid

#216154

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-FLAG

Plasmid

#205619

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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