Addgene

We narrowed to 1,961 results for: IPP

Showing: 181 - 210 of 1961 results

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pAAV-hSyn-DD-T6B-WT-YFP

Plasmid

#235143

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

Plasmid

#235158

Purpose

Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/primary transcript with control siRNA

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-DD-T6B-WT-YFP

Plasmid

#235145

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Tags

Expression

Mutation

Promoter

L7-6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-WT-YFP

Plasmid

#235141

Purpose

Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Expression

Mutation

Promoter

L7-6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLAG-HA-T6B-WT-YFP

Plasmid

#235139

Purpose

Expresses the T6B peptide fused with YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Expression

Mutation

Promoter

hSyn

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-HaloTag-linker-SpyCatcher3

Plasmid

#235150

Purpose

Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

HaloTag linked to SpyCatcher3

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAHW/Joseph2-Multitagged Plasmid

Plasmid

#226725

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pAHW/Joseph2

Use

Drosophila s2 cell expression

Tags

HA, Myc, GFP, Flag, V5, 6xHis

Expression

Insect

Mutation

Promoter

Act5C

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMH-HA/Joseph2-Multitagged Plasmid

Plasmid

#226726

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pMH-HA/Joseph2

Use

Tags

HA, Myc, GFP, Flag, V5, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Tol2 8xSTAR-sTomato. PGK-puro

Plasmid

#136264

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAGH50

Plasmid

#107254

Purpose

Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp-fkbp12

Use

Suicide vector for destined for double homologous…

Tags

GFP and FKBP12

Expression

Bacterial

Mutation

Promoter

native

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-VHL(1-155)

Plasmid

#29500

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Use

Tags

Expression

Insect

Mutation

VHL(1-155)only

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-Sec23A

Plasmid

#166894

Purpose

mammalian expression of Sec23A tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec23A

Plasmid

#166893

Purpose

mammalian expression of Sec23A tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Q71L

Promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSVG

Plasmid

#11912

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

F64L, S65T, A206K relative the wild type GFP sequ…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-Sec31

Plasmid

#166895

Purpose

mammalian expression of Sec31 tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sec31

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP-VP16

Plasmid

#183923

Purpose

VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-VP16

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-MS2coatProtein

Plasmid

#103832

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mCherry-MS2 coat protein (cp )

Use

Tags

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-dCas9-HA-NLS--GFP-p300

Plasmid

#183925

Purpose

p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-p300

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

Plasmid

#136262

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2 8xSTAR-sTomato-NLS. PGK-puro

Plasmid

#136261

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Sar1b-H79G-YFP

Plasmid

#166898

Purpose

mammalian expression of Sar1b-H79G tagged with YFP

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sar1b-H79G

Use

Tags

YFP

Expression

Mammalian

Mutation

H79 to G

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVenus-VSVG

Plasmid

#11914

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
ECFP-GalT

Plasmid

#11937

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAGFP-VSVG

Plasmid

#11915

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

(L64F/T65S/V163A/T203H). The PAGFP also contains …

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-Sec23A

Plasmid

#166892

Purpose

mammalian expression of Sec23A tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ptagRFP-MS2coatProtein

Plasmid

#103831

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

MS2 coat protein (MCP) (cp )

Use

Tags

tagRFP

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Use

Tags

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Sar1b-H79G-mOrange2

Plasmid

#166900

Purpose

mammalian expression of Sar1b-H79G tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sar1b

Use

Tags

mOrange2

Expression

Mammalian

Mutation

H79G

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge