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We narrowed to 2,347 results for: puc19

Showing: 301 - 350 of 2347 results

  3. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid
    #226989
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1a to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226989-plasmid-map-sequence-id-453964

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  4. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid
    #226991
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2a to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226991-plasmid-map-sequence-id-453954

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  7. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid
    #226992
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226992-plasmid-map-sequence-id-451253

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  8. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid
    #226990
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226990-plasmid-map-sequence-id-451250

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  14. pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

    Plasmid
    #110408
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110408-plasmid-map-sequence-id-218853

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  19. Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169353
    Purpose
    Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Insert
    OsTIR1(F74A)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F74A
    Promoter
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169353-plasmid-map-sequence-id-332957

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  20. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  23. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  24. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  25. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  28. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    Available Since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

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  29. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available Since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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  35. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  36. pMT440

    Plasmid
    #8609
    Depositor
    Robert Hartley
    Article
    Yazynin et al Gene 1996 Feb 22;169(1):131-2.
    Insert
    barnase, barstar
    Use
    Tags
    as pMT416
    Expression
    Bacterial
    Mutation
    V36 codon of barnase of pMT416 replaced by polyli…
    Promoter
    Available Since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  37. pSelAct-Express

    Plasmid
    #174375
    Purpose
    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Expression vector for targeted integration
    Tags
    Expression
    Mutation
    Promoter
    pCMP1
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    174375-plasmid-map-sequence-id-370623

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  38. pSelAct-KO

    Plasmid
    #174374
    Purpose
    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Vector for 2-step knockout by homologous recombin…
    Tags
    Expression
    Mutation
    Promoter
    N/A
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174374-plasmid-map-sequence-id-356074

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  39. pHB1

    Plasmid
    #204668
    Purpose
    pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs
    Depositor
    Mark Mandel
    Article
    Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do
    Insert
    FRT-erm-FRT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    204668-plasmid-map-sequence-id-422775

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  40. pCVD432

    Plasmid
    #63755
    Purpose
    Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.
    Depositor
    James Kaper
    Article
    Baudry et al J Infect Dis. 1990 Jun;161(6):1249-51.
    Insert
    EAggEC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  41. pILGFP1DFB

    Plasmid
    #185871
    Purpose
    A parental plasmid for in vivo gene amplification at RPL25 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185871-plasmid-map-sequence-id-370815

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  43. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  44. pMT1004

    Plasmid
    #8613
    Depositor
    Robert Hartley
    Insert
    binase, barstar
    Use
    Tags
    Expression
    Bacterial
    Mutation
    as pMT1002
    Promoter
    Available Since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    8613-plasmid-map-sequence-id-240677

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  45. pILGFP4BG6

    Plasmid
    #185877
    Purpose
    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185877-plasmid-map-sequence-id-370820

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  46. pILGFP3AA5

    Plasmid
    #185875
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185875-plasmid-map-sequence-id-369502

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  47. pILGFP3AG4

    Plasmid
    #185874
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185874-plasmid-map-sequence-id-370818

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  48. pILGFP5EA4

    Plasmid
    #185879
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185879-plasmid-map-sequence-id-370824

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  49. pILGFP5EC4

    Plasmid
    #185880
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185880-plasmid-map-sequence-id-370826

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  50. pILGFP5EF3

    Plasmid
    #185881
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185881-plasmid-map-sequence-id-370828

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Showing: 301 - 350 of 2347 results