We narrowed to 34,850 results for: CaS

1. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

   Plasmid

   #211767

   Purpose

   dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pGK and pEF1a

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

   Plasmid

   #217801

   Purpose

   Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Plasmid

   #101230

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Plasmid

   #101231

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Plasmid

   #101229

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

   Plasmid

   #189636

   Purpose

   Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain

   Plasmid

   #183396

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain

   Plasmid

   #183395

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

   Plasmid

   #219823

   Purpose

   EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

   Depositor

   Philipp Maass

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid

    #191795

    Purpose

    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    A134V, A140V, A141V, A143V

    Promoter

    EFS, hU6

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid

    #182360

    Purpose

    Shuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2

    Depositor

    Mario Capecchi

    Insert

    Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiV_Cas9_Blast

    Plasmid

    #125592

    Purpose

    Lentiviral expression of spCas9 with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonor_ShCAST_kanR

    Plasmid

    #127924

    Purpose

    Kanamycin resistance donor for ShCAST

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MbCas12a_N1153_GPG_GR2

    Plasmid

    #231578

    Purpose

    Encodes a cortisol inducible variant of MbCas12a

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    MbCas12a with a GR2 insertion behind N1153
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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