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Showing: 121 - 140 of 227 results

  16. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  17. mr-GST full length

    Plasmid
    #112982
    Purpose
    For protein expression and purification of full-length Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  18. mr-GST N-term fusion

    Plasmid
    #112983
    Purpose
    For protein expression and purification of N-terminus of Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-term only
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112983-plasmid-map-sequence-id-217464

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  20. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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Showing: 121 - 140 of 227 results