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Showing: 121 - 140 of 1125 results

  14. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid
    #183073
    Purpose
    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression
    Depositor
    Jennifer Doudna
    Article
    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4
    Insert
    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183073-plasmid-map-sequence-id-358157

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  16. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176255-plasmid-map-sequence-id-347038

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  17. pNOC_hfnCas12a_crRNA NR2

    Plasmid
    #176248
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176248-plasmid-map-sequence-id-347016

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  18. pNOC_hfnCas12a_crRNA NR1

    Plasmid
    #176247
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176247-plasmid-map-sequence-id-346571

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  19. pNOC_hfnCas12a_crRNA NR3

    Plasmid
    #176249
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    176249-plasmid-map-sequence-id-347037

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Showing: 121 - 140 of 1125 results