We narrowed to 2,209 results for: cas12

1. Cas12j-8-puro

   Plasmid

   #194966

   Purpose

   The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ133-RZ-Cas12j2

   Plasmid

   #189784

   Purpose

   Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV261-Cas12a

   Plasmid

   #158707

   Purpose

   expresses codon-optimized FnCpf1

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCC_039 FnCas12a

   Plasmid

   #171666

   Purpose

   For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC_hfnCas12a-Nlux

   Plasmid

   #176239

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Ribosomal subunit

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET47b-T7-AsCas12f-WT

   Plasmid

   #204640

   Purpose

   Expression of wild-type AsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   AsCas12f
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-AsCas12f-WT

   Plasmid

   #204635

   Purpose

   Expression of wild-type AsCas12f in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   AsCas12f
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_hlbCas12a-Nlux

    Plasmid

    #176240

    Purpose

    pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized lbCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Ribosomal subunit

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas12f-GE ver4.0

    Plasmid

    #176543

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.0
    Cas12f-GE ver4.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ-NF-L3-NF-L1-dMb2Cas12a

    Plasmid

    #177686

    Purpose

    Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    2xFokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJH019_SC101_pLAC_Cas12c(4)_Kan

    Plasmid

    #183071

    Purpose

    Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Wild-type

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas12j2 KRAB

    Plasmid

    #188508

    Purpose

    Expresses FLAG tagged dCas12j2 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ281 (MbCas12a)

    Plasmid

    #138113

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    MbCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas12f-GE ver3.0

    Plasmid

    #176508

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver3.0
    Cas12f-GE ver3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AsCas12a-DR-cr in PBCSK-

    Plasmid

    #126640

    Purpose

    Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJH059_proD_Cas12c_DualRNA-GFP_CAM

    Plasmid

    #183076

    Purpose

    Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and pre-crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ284 (Mb2Cas12a)

    Plasmid

    #138116

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Mb2Cas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge