We narrowed to 235 results for: stem loop

1. pSP266

   Plasmid

   #139488

   Purpose

   Insertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pCTT1 24xPP7sl
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pCTT1

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSP264

   Plasmid

   #139487

   Purpose

   Insertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pSTL1 24xPP7sl
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pSTL1

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVW200

   Plasmid

   #139489

   Purpose

   Insertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pGPD1 24xPP7sl
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pGPD1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVW293

   Plasmid

   #139490

   Purpose

   Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pHSP12 24xPP7sl
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pHSP12

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SL-SunTag

   Plasmid

   #196919

   Purpose

   The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC57-PP7v3

   Plasmid

   #107230

   Purpose

   Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   24xPP7v3
   

   Use

   Tags

   3x FLAG-tag and P2A site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRPa-iSL(MCS1+2)

   Plasmid

   #69244

   Purpose

   Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   Expression

   Mutation

   Promoter

   RRNA promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Let7-SunTag

   Plasmid

   #196928

   Purpose

   Let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Let7R-SunTag

   Plasmid

   #196929

   Purpose

   Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRPa-iSL(MCS3+4)

    Plasmid

    #69246

    Purpose

    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    Expression

    Mutation

    Promoter

    RRNA promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFH3.2_mTagBFP2-MS2x12

    Plasmid

    #205558

    Purpose

    Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFH2.22_mCherry-MS2x8

    Plasmid

    #205537

    Purpose

    Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH2.214_mCherry-PP7x12

    Plasmid

    #205556

    Purpose

    Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFH2.19_mCherry-MS2x2

    Plasmid

    #205534

    Purpose

    Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NLS-tdPCP-GFP

    Plasmid

    #183934

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFH2.20_mCherry-MS2x4

    Plasmid

    #205535

    Purpose

    Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge