We narrowed to 1,597 results for: tef

1. pRS415-TEF1pr-SEC61-FAPoptim

   Plasmid

   #221115

   Purpose

   FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   SEC61-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS413-TEF1pr-MFA-NFAPoptim

   Plasmid

   #221095

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS415-TEF1pr-STE3-GFP

   Plasmid

   #221113

   Purpose

   GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   STE3-GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS413-TEF1pr-ANP1-FAPoptim

   Plasmid

   #221120

   Purpose

   FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   ANP1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS413-TEF1pr-ERG6-FAPoptim

   Plasmid

   #221121

   Purpose

   FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   ERG6-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS413-TEF1pr-PMA1-FAPoptim

   Plasmid

   #221122

   Purpose

   FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   PMA1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS413-TEF1pr-RPA34-FAPoptim

   Plasmid

   #221123

   Purpose

   FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   RPA34-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS413-TEF1pr-SEC7-FAPoptim

   Plasmid

   #221124

   Purpose

   FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   SEC7-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS413-TEF1pr-VPH1-FAPoptim

   Plasmid

   #221125

   Purpose

   FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   VPH1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIplac128-TEF2p-LDH-CYC1t

    Plasmid

    #216120

    Purpose

    Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Lactate dehydrogenase (LDH Bovine)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF2 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

    Plasmid

    #139963

    Purpose

    Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

    Plasmid

    #139964

    Purpose

    Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416-PTEF1-MCP-3P-ECM33_intron

    Plasmid

    #127601

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 3′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS416-PTEF1-MCP-5M-ECM33_intron

    Plasmid

    #127600

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS416-PTEF1-MCP-5P-ECM33_intron

    Plasmid

    #127599

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS416-PTEF1-MCP-ECM33_intron

    Plasmid

    #127598

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS416-PTEF1-MCP-no_intron

    Plasmid

    #127597

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    1xFLAG-MCP expression cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDSM-bc-Psmtef1-CAD-Tyhi9

    Plasmid

    #127730

    Purpose

    Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Psmtef1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge