We narrowed to 2,465 results for: Kap

1. pcDNA3 IKKe flag

   Plasmid

   #26201

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon (IKBKE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.4_1G11F2-3 light chain

   Plasmid

   #198254

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-TRIM28deltaLinker

   Plasmid

   #124958

   Purpose

   Mammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domains

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of linker region between CC domain and P…

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti_CMV:Flag-OeNL

   Plasmid

   #208850

   Purpose

   Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   OeNL Nanolantern
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Mito-CaNAR2

   Plasmid

   #64731

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal 30 amino acids from DAKAP1,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET15b/bPanK

   Plasmid

   #73619

   Purpose

   Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

   Depositor

   Suzanne Jackowski, T. J. Kappock

   Article

   Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

   Insert

   pantothenate kinase (coaA Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   relative to MG1655 sequence, changes glutamate-5 …

   Promoter

   T7

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAPM-D4 miR30-TRIM28 ts3

   Plasmid

   #115864

   Purpose

   TRIM28 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   TRIM28 shRNA (TRIM28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK7 IKK2/IKKb

   Plasmid

   #26203

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase beta (IKBKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-FRISZ-TM

    Plasmid

    #176886

    Purpose

    AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    Ig kappa-FRISZ-PDGFR TM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

    Plasmid

    #173073

    Purpose

    Dead SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    AA144 in both SNAP tags changed from a cysteine t…

    Promoter

    CMV IE94

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSVE Grb2 myc 49L

    Plasmid

    #26085

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 P49L (GRB2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    P49L

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. ch-Tog

    Plasmid

    #28290

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    ch-TOG (CKAP5 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    K19R, E118G

    Promoter

    Available since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3 IKKe T7

    Plasmid

    #27238

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pSFG-TFII-I-GFP beta

    Plasmid

    #22200

    Depositor

    Ananda Roy

    Article

    Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eDrTrkA

    Plasmid

    #183996

    Purpose

    Optically controlled eDrTrkA expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkA (NTRK1 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSFG-TFII-I-GFP delta

    Plasmid

    #22190

    Depositor

    Ananda Roy

    Article

    Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.4_7C11-1 light chain

    Plasmid

    #198252

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Nap4-SH2

    Plasmid

    #46456

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nap4 (SOCS7 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 395-514

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZac2.1-GfaABC1D -IgK- TurboID- NCAN IL-HA

    Plasmid

    #219808

    Purpose

    Secreted HA-tagged TurboID-NCAN-IL domain for extracellular proximity labeling

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    IgK (Igk Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid

    #196973

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GFP and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid

    #196976

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

    Depositor

    Lukas Kapitein

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GST and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eDrTrkB

    Plasmid

    #183997

    Purpose

    Optically controlled eDrTrkB expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET12a-AL-103 Y32F Y96F

    Plasmid

    #47092

    Depositor

    Marina Ramirez-Alvarado

    Article

    DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

    Insert

    AL-103 Y32F Y96F (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Y32F Y96F

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pEBG-TFII-I L30&37P

    Plasmid

    #22166

    Depositor

    Ananda Roy

    Article

    Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 30 to Proline and changed leucine…

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

27. GST-PS-Grb2 (XSB363)

    Plasmid

    #78505

    Purpose

    Construct for expressing Grb2 in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. OMM-V5-LOV-Turbo

    Plasmid

    #199665

    Purpose

    expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    AKAP1 transmembrane domain and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBMN-HA-TBK1

    Plasmid

    #199205

    Purpose

    Used for the expression of TBK1 (SMC Internal No. 1996)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-scFv-T

    Plasmid

    #67843

    Purpose

    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

    Depositor

    Joanna Bereta

    Article

    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

    Insert

    antibody 1D8 light kappa chain
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge