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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,030 results for: Nes

Showing: 1381 - 1400 of 25030 results

  1. K980

    Plasmid
    #129762
    Purpose
    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129762-plasmid-map-sequence-id-263587

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  2. K406

    Plasmid
    #129764
    Purpose
    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 406
    Promoter
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129764-plasmid-map-sequence-id-263574

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  3. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Promoter
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  4. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  5. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  6. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  7. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129761-plasmid-map-sequence-id-422377

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  8. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Promoter
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  18. JPF0419d

    Plasmid
    #124020
    Purpose
    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124020-plasmid-map-sequence-id-262655

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  19. JPF0419v

    Plasmid
    #124021
    Purpose
    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124021-plasmid-map-sequence-id-262675

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Showing: 1381 - 1400 of 25030 results