We narrowed to 6,488 results for: rel

1. pDONR221-EWSR1 g1750a Stop

   Plasmid

   #84893

   Purpose

   DONOR vector for Gateway cloning of EWSR1 g1750a Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   g1750a

   Promoter

   none

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221-EWSR1 c1655t Stop

   Plasmid

   #84891

   Purpose

   DONOR vector for Gateway cloning of EWSR1 c1655t Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   c1655t

   Promoter

   none

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. CMV-mito-R-GECO1

   Plasmid

   #46021

   Purpose

   Red intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   R-GECO1
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Myo10

   Plasmid

   #135403

   Purpose

   Expresses GFP-tagged bovine Myo10 in mammalian cells.

   Depositor

   Richard Cheney

   Article

   Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

   Insert

   Myo10 (MYO10 Bovine)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   "Relative to the GenBank Myo10 cDNA sequence…

   Promoter

   CMV

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6/V5-DEST-RINT1

   Plasmid

   #40124

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   RINT1 (RINT1 Human)
   

   Use

   Lentiviral

   Tags

   V5 epitope

   Expression

   Mammalian

   Mutation

   L270M relative to NM_021930

   Promoter

   CMV

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDN-D2irTNG4kwh

   Plasmid

   #44722

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   htetR::NLS::EGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter;…

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. LSB-hsa-miR-92a-1-5p

   Plasmid

   #103750

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-92a-1-5p target (MIR92A1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGG442

   Plasmid

   #165616

   Purpose

   Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
   

   Use

   CRISPR

   Tags

   3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

   Expression

   Yeast

   Mutation

   Corrected homology relative to wild type SpCas9 (…

   Promoter

   TEF1

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-BBclone1

    Plasmid

    #137069

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. hIgG1_heavy_CoVMab7

    Plasmid

    #194605

    Purpose

    Mammalian expression of CoVMab7 heavy chain

    Depositor

    Ben Murrell

    Insert

    CoVMab7 heavy chain (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hIgG1_heavy_CoVMab4

    Plasmid

    #194603

    Purpose

    Mammalian expression of CoVMab4 heavy chain

    Depositor

    Ben Murrell

    Insert

    CoVMab4 heavy chain (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hIgG1_kappa_CoVMab7

    Plasmid

    #194606

    Purpose

    Mammalian expression of CoVMab7 light chain

    Depositor

    Ben Murrell

    Insert

    CoVMab7 light chain (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. hIgG1_kappa_CoVMab4

    Plasmid

    #194604

    Purpose

    Mammalian expression of CoVMab4 light chain

    Depositor

    Ben Murrell

    Insert

    CoVMab4 light chain (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKPY738

    Plasmid

    #72674

    Purpose

    C. elegans let-858 3' UTR (Gateway Entry Clone)

    Depositor

    David Tirrell

    Insert

    let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKPY115

    Plasmid

    #72669

    Purpose

    Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control

    Depositor

    David Tirrell

    Insert

    Thr251Gly-EcPheRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr251Gly in Alpha Subunit

    Promoter

    PBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. hIgG1-F1-Fc

    Plasmid

    #194597

    Purpose

    Mammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Ben Murrell

    Insert

    F1-Fc (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. hIgG1-E2-Fc

    Plasmid

    #194595

    Purpose

    Mammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Ben Murrell

    Insert

    E2-Fc (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. hIgG1-G6-Fc

    Plasmid

    #194594

    Purpose

    Mammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Ben Murrell

    Insert

    G6-Fc (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hIgG1-C7-Fc

    Plasmid

    #194596

    Purpose

    Mammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Ben Murrell

    Insert

    C7-Fc (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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