We narrowed to 8,504 results for: sgrna

1. lentiCRISPR - DDX3Y sgRNA 2

   Plasmid

   #70657

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR - DDX3Y sgRNA 1

   Plasmid

   #70656

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. sgRNA BRCA2 V572I

   Plasmid

   #139321

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 V572I using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA1_GAL4UAS-Luciferase reporter

   Plasmid

   #64157

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330-UFSP2 sgRNA2

   Plasmid

   #134637

   Purpose

   contains sgRNA targeting human UFSP2 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFSP2 sgRNA2 (UFSP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

   Plasmid

   #81246

   Purpose

   For assaying self-targeting activity via Cas9

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   sgRNA
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA2_GAL4UAS-Luciferase reporter

   Plasmid

   #64158

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMulti-sgRNA-LacZ

   Plasmid

   #99913

   Purpose

   Receptor plasmid for the assembly of multiple sgRNAs

   Depositor

   Yujie Sun

   Article

   Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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