We narrowed to 1,917 results for: FRT

1. BirA-Myc_N_SP1

   Plasmid

   #167726

   Purpose

   Expresses SP1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   SP1 (SP1 Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Endoplasmic reticulum-targeted optodPLD

   Plasmid

   #140059

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Use

   Tags

   CIBN, CRY2, P2A, Sac1(TM), and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Promoter

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-hygro-eGFP-HEC1-9A

   Plasmid

   #114028

   Purpose

   Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 9A

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV plasmid

   Plasmid

   #169739

   Purpose

   Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDREV-2

   Plasmid

   #26752

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   H2B-Venus CDS (H2BC3 Human)
   

   Use

   Cre/Lox; Flp/frt; dre/rox

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. FFiBlast p5

   Plasmid

   #68470

   Purpose

   Inverted and floxed blasticidin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKF-P14MM2AG5h

   Plasmid

   #128254

   Purpose

   Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-puro-eGFP-HEC1-9A

   Plasmid

   #114023

   Purpose

   Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 9A

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDREV-0

   Plasmid

   #26750

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   H2B-Venus CDS (H2BC3 Human)
   

   Use

   Cre/Lox; Flp/frt; dre/rox

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-hygro-eGFP-HEC1-9D

    Plasmid

    #114029

    Purpose

    Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9D

    Promoter

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDREV-1

    Plasmid

    #26751

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    H2B-Venus CDS (H2BC3 Human)
    

    Use

    Cre/Lox; Flp/frt; dre/rox

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-hygro-eGFP-HEC1-8A/S55D

    Plasmid

    #114032

    Purpose

    Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 8A/S55D

    Promoter

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPYRMandi-ABI

    Plasmid

    #233649

    Purpose

    Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

    Depositor

    Richard Wombacher

    Article

    Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

    Insert

    TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDN-D2irTNG5kwh

    Plasmid

    #128279

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion protein

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::NLS::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-puro-eGFP-HEC1-d80

    Plasmid

    #114025

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-hygro-eGFP-HEC1-d80

    Plasmid

    #114030

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-hygro-eGFP-HEC1-8A/S15D

    Plasmid

    #114031

    Purpose

    Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 8A/S15D

    Promoter

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FR_GFP

    Plasmid

    #31302

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. dRMCE Plasmids

    Plasmid Kit

    #1000000015

    Purpose

    Takes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous loci

    Depositor

    Rolf Zeller

    Available Since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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