We narrowed to 7,595 results for: Ski;

1. AAVS1-Neo-TRE-CMV-Cre-rtTA

   Plasmid

   #165457

   Purpose

   Tetracycline inducible CRE expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   CRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA1 Flag ATXN1[85Q]

   Plasmid

   #33237

   Depositor

   Huda Zoghbi

   Article

   Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 85 Glutamines and no…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET26b_AT118-L-V5-His

   Plasmid

   #222848

   Purpose

   E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCXIP-GFP-RPA70

   Plasmid

   #164231

   Purpose

   retroviral plasmid for GFP-RPA70

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   RPA70 (RPA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA1 Flag ATXN1[30Q]

   Plasmid

   #33236

   Depositor

   Huda Zoghbi

   Article

   Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 29 amino acids (27 Q…

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pBABEpuro-ARAF

   Plasmid

   #51076

   Purpose

   mammalian expression of wt ARAF

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX302 OASL-V5 puro

   Plasmid

   #158643

   Purpose

   Lentiviral expression vector for constitutive expression of V5-tagged OASL.

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   OASL (OASL Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX302 OAS1-V5 puro

   Plasmid

   #158641

   Purpose

   Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   OAS1 (OAS1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102889

   Purpose

   PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA1 Flag ATXN1[2Q]

    Plasmid

    #33235

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    CMV

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWPI-GFP-3xFLAG

    Plasmid

    #201639

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAS9PBKS

    Plasmid

    #68371

    Purpose

    CbAkt promoter driven Cas9-2A-GFP (from px458)

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    Cas9
    

    Use

    Tags

    2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CbAkt

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRARE-MBP-DEST

    Plasmid

    #84650

    Purpose

    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

    Depositor

    Neil Olszewski

    Article

    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p205-5'-Switch-ON

    Plasmid

    #217884

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB9v1

    Plasmid

    #210028

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6GRNA

    Plasmid

    #68370

    Purpose

    gRNA scaffold with hU6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEP300-pAAV-CMV-MCS2-pA

    Plasmid

    #112138

    Purpose

    CMV driven Multi Cloning Site-2

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGG-NFIX2

    Plasmid

    #112699

    Purpose

    Expression of NFIX2 protein and GFP

    Depositor

    Francois Guillemot, Richard Gronostajski

    Article

    Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

    Insert

    Nuclear Factor I X2 (Nfix Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-Cluc-RMA

    Plasmid

    #189624

    Purpose

    Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    Cypridina luciferase fused to Fc
    

    Use

    AAV and Luciferase

    Tags

    Cluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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