Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,295 results for: genome

Clear keywords
Showing: 1401 - 1420 of 16295 results

  5. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

    Enlarge

  8. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid
    #136652
    Purpose
    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136652-plasmid-map-sequence-id-273328

    Enlarge

  10. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

    Enlarge

  13. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid
    #136657
    Purpose
    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136657-plasmid-map-sequence-id-273344

    Enlarge

  14. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid
    #136654
    Purpose
    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136654-plasmid-map-sequence-id-273340

    Enlarge

  16. 4QOX

    Plasmid
    #61959
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-CDPK4
    Use
    Baculovirus expression
    Tags
    MHHHHHHSSGRENLYFQG
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available Since
    May 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. PP-ClpR

    Plasmid
    #32876
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ClpR (PF3D7_1436800 Plasmodium Falciparum)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Jan. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

    Enlarge

  19. pK7-g7g8-K8

    Plasmid
    #218220
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218220-plasmid-map-sequence-id-432226

    Enlarge

  20. pK3-g3g4-K4

    Plasmid
    #218218
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218218-plasmid-map-sequence-id-432228

    Enlarge

Showing: 1401 - 1420 of 16295 results